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FEBS Lett. 2015 Apr 2;589(8):933-40. doi: 10.1016/j.febslet.2015.02.025. Epub 2015 Mar 3.

Identification of minimum Rpn4-responsive elements in genes related to proteasome functions.

Author information

1
Laboratory of Protein Metabolism, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan.
2
Laboratory of Protein Metabolism, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo 113-0033, Japan. Electronic address: smurata@mol.f.u-tokyo.ac.jp.

Abstract

The proteasome is an essential, 66-subunit protease that mediates ubiquitin-dependent proteolysis. The transcription factor Rpn4 regulates concerted expression of proteasome subunits to increase the proteasome by recognizing nonamer proteasome-associated control element (PACE) elements on the promoter regions. However, the genes for proteasome assembly chaperones and some of the subunits have no PACEs. Here we identified a minimal hexamer "PACE-core" sequence that responds to Rpn4. PACE-cores are found in many genes related to proteasome function including the assembly chaperones, but cannot substitute for PACE of the subunits. Our results add a new layer of complexity in transcriptional regulation of genes involved in protein degradation.

KEYWORDS:

Proteasome; Rpn4; Transcriptional regulation

PMID:
25747386
DOI:
10.1016/j.febslet.2015.02.025
[Indexed for MEDLINE]
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