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Mol Cancer. 2015 Feb 22;14:51. doi: 10.1186/s12943-015-0318-0.

The long noncoding RNA SPRY4-IT1 increases the proliferation of human breast cancer cells by upregulating ZNF703 expression.

Author information

1
Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China. 1138303166@qq.com.
2
Taixing People's Hospital, Taixing, Jiangsu, PR China. 1138303166@qq.com.
3
Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China. lijuan123@163.com.
4
Taixing People's Hospital, Taixing, Jiangsu, PR China. liuyctx@163.com.
5
Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China. 430397952@qq.com.
6
Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China. yingrui668@163.com.
7
Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China. 82560036@qq.com.
8
Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China. 911165@qq.com.
9
Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China. Yifan23@sohu.com.
10
Department of Oncology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China. kemingwang@njmu.edu.cn.
11
Department of Oncology, the First Affiliated Hospital of Nanjing Medical University, Nanjing, Jiangsu, PR China. shuyongqian@csco.org.cn.

Abstract

BACKGROUND:

Long noncoding RNAs (lncRNAs) have emerged recently as a new class of genes that regulate cellular processes, such as cell growth and apoptosis. The SPRY4 intronic transcript 1 (SPRY4-IT1) is a 708-bp lncRNA on chromosome 5 with a potential functional role in tumorigenesis. The clinical significance of SPRY4-IT1 and the effect of SPRY4-IT1 on cancer progression are unclear.

METHODS:

Quantitative reverse transcriptase PCR (qRT-PCR) was performed to investigate the expression of SPRY4-IT1 in 48 breast cancer tissues and four breast cancer cell lines. Gain and loss of function approaches were used to investigate the biological role of SPRY4-IT1 in vitro. Microarray bioinformatics analysis was performed to identify the putative targets of SPRY4-IT1, which were further verified by rescue experiments, and by western blotting and qRT-PCR.

RESULTS:

SPRY4-IT1 expression was significantly upregulated in 48 breast cancer tumor tissues comparedwith normal tissues. Additionally, increased SPRY4-IT1 expression was found to be associated with a larger tumor size and an advanced pathological stage in breast cancer patients. The knockdown of SPRY4-IT1 significantly suppressed proliferation and caused apoptosis of breast cancer cells in vitro. Furthermore, we discovered that ZNF703 was a target of SPRY4-IT1 and was downregulated by SPRY4-IT1 knockdown. Moreover, we provide the first demonstration that ZNF703 plays an oncogenic role in ER (-) breast carcinoma cells.

CONCLUSIONS:

SPRY4-IT1 is a novel prognostic biomarker and a potential therapeutic candidate for breast cancer.

PMID:
25742952
PMCID:
PMC4350857
DOI:
10.1186/s12943-015-0318-0
[Indexed for MEDLINE]
Free PMC Article

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