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J Periodontol. 2015 Jun;86(6):801-11. doi: 10.1902/jop.2015.140630. Epub 2015 Mar 5.

Effects of electroacupuncture on experimental periodontitis in rats.

Author information

1
*Department of Clinical Dentistry, Federal University of Ceará, Fortaleza, Ceará, Brazil.
2
†Department of Morphology, Faculty of Medicine, Federal University of Ceará.
3
‡Department of Basic Sciences, Division of Histology, Dental School of Aracatuba, São Paulo State University, Aracatuba, São Paulo, Brazil.
4
§Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará.
5
‖Department of Oral and Maxillofacial Surgery and Periodontology, Ribeirao Preto School of Dentistry, University of São Paulo, Ribeirao Preto, São Paulo, Brazil.
6
¶Department of Veterinary Pathology, São Paulo State University, Jaboticabal, São Paulo, Brazil.

Abstract

BACKGROUND:

Acupuncture has shown the capability of modulating the immuno-inflammatory response of the host. This study aims to evaluate the effects of electroacupuncture (EA) on ligature-induced periodontitis in rats.

METHODS:

Thirty-two animals were divided into four groups: 1) control; 2) experimental periodontitis (EP); 3) sham-treated (EP/EA-sham); and 4) treated with EA (EP/EA). For the EP groups, a ligature was placed around the right mandibular first molars at day 1. Sessions of EA or EA-sham were assigned every other day. For EA treatment, large intestine meridian points LI4 and LI11 and stomach meridian points ST36 and ST44 were used. EA-sham was performed in off-meridian points. Animals were euthanized at day 11. Histomorphometric and microtomographic analyses were performed. Immunolabeling patterns for the receptor activator of nuclear factor κB ligand (RANKL), osteoprotegerin (OPG), and tartrate-resistant acid phosphatase (TRAP) were assessed. Expressions of interleukin (IL)-1β, matrix metalloproteinase (MMP)-8, IL-6, and cyclooxygenase (COX)-2 messenger RNAs (mRNAs) were evaluated by quantitative reverse transcription-polymerase chain reaction. Data were analyzed statistically (P <0.05, analysis of variance).

RESULTS:

Histomorphometric and microtomographic analyses demonstrated that group EP/EA presented reduced alveolar bone loss when compared to group EP (P <0.05). Reduced RANKL immunolabeling and fewer TRAP-positive multinucleated cells were observed in the EA-treated group in relation to group EP. No differences were observed in OPG expression among groups. EA treatment decreased the genic expression of IL-1β and MMP-8 (P <0.05), increased the mRNA expression of IL-6 (P <0.05), and did not modify the genic expression of COX-2 in animals with EP (P >0.05).

CONCLUSION:

It can be concluded that EA reduced periodontal tissue breakdown and the expression of some proinflammatory mediators and a proresorptive factor in EP in rats.

KEYWORDS:

Bone resorption; electroacupuncture; inflammation mediators; periodontitis.

PMID:
25741581
DOI:
10.1902/jop.2015.140630
[Indexed for MEDLINE]

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