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J Exp Bot. 2015 Apr;66(8):2283-92. doi: 10.1093/jxb/erv006. Epub 2015 Mar 4.

Phenotyping pipeline reveals major seedling root growth QTL in hexaploid wheat.

Author information

1
Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Sutton Bonington LE12 5RD, UK.
2
Department of Crop Genetics, John Innes Centre, Norwich Research Park, Norwich NR4 7UH, UK.
3
Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Sutton Bonington LE12 5RD, UK.
4
INRA, UMR 1095 Génétique, Diversité et Ecophysiologie des Céréales, 63100 Clermont-Ferrand, France.
5
Division of Plant and Crop Sciences, School of Biosciences, University of Nottingham, Sutton Bonington LE12 5RD, UK j.king@nottingham.ac.uk darren.wells@nottingham.ac.uk.
6
Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Sutton Bonington LE12 5RD, UK j.king@nottingham.ac.uk darren.wells@nottingham.ac.uk.

Abstract

Seedling root traits of wheat (Triticum aestivum L.) have been shown to be important for efficient establishment and linked to mature plant traits such as height and yield. A root phenotyping pipeline, consisting of a germination paper-based screen combined with image segmentation and analysis software, was developed and used to characterize seedling traits in 94 doubled haploid progeny derived from a cross between the winter wheat cultivars Rialto and Savannah. Field experiments were conducted to measure mature plant height, grain yield, and nitrogen (N) uptake in three sites over 2 years. In total, 29 quantitative trait loci (QTLs) for seedling root traits were identified. Two QTLs for grain yield and N uptake co-localize with root QTLs on chromosomes 2B and 7D, respectively. Of the 29 root QTLs identified, 11 were found to co-localize on 6D, with four of these achieving highly significant logarithm of odds scores (>20). These results suggest the presence of a major-effect gene regulating seedling root vigour/growth on chromosome 6D.

KEYWORDS:

High-throughput phenotyping; root system architecture.

PMID:
25740921
PMCID:
PMC4407652
DOI:
10.1093/jxb/erv006
[Indexed for MEDLINE]
Free PMC Article

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