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Pathog Dis. 2015 Jul;73(5). pii: ftv016. doi: 10.1093/femspd/ftv016. Epub 2015 Mar 2.

Potentiation of ciprofloxacin action against Gram-negative bacterial biofilms by a nitroxide.

Author information

1
Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC V6T 1Z4, Canada.
2
ARC Centre of Excellence for Free Radical Chemistry and Biotechnology, Faculty of Science and Engineering, Queensland University of Technology, Queensland 4001, Australia.
3
Centre for Microbial Diseases and Immunity Research, Department of Microbiology and Immunology, University of British Columbia, Vancouver, BC V6T 1Z4, Canada bob@hancocklab.com.

Abstract

We previously showed that soluble nitroxides (nitric oxide analogues) mimicked the well-established ability of nitric oxide to cause biofilm dispersal and further showed that these compounds could prevent biofilm formation. Here, we investigated the effect of the nitroxide carboxy-TEMPO in combination with sub μg/ml concentrations of ciprofloxacin on pre-formed flow cell biofilms formed by Gram-negative bacteria. Combination therapy led to substantial eradication of existing biofilms formed by Pseudomonas aeruginosa PA14 (99.3%) and Escherichia coli O157 (93%).

KEYWORDS:

biofilm; ciprofloxacin; nitroxide

PMID:
25736816
DOI:
10.1093/femspd/ftv016
[Indexed for MEDLINE]

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