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Proteomics Clin Appl. 2015 Jun;9(5-6):543-51. doi: 10.1002/prca.201400205. Epub 2015 Apr 17.

Detection of urinary biomarkers in reservoir hosts of leptospirosis by capillary electrophoresis-mass spectrometry.

Author information

1
School of Veterinary Medicine, University College Dublin, Belfield, Dublin, Ireland.
2
BHF Glasgow Cardiovascular Research Centre, Institute of Cardiovascular and Medical Sciences, College of Medical Veterinary and Life Sciences, University of Glasgow, Glasgow, UK.
3
Ross University School of Medicine, St. Kitts, West Indies.
4
Mosaiques diagnostics, Hannover, Germany.
5
School of Natural Sciences, University of Stirling, Stirling, UK.

Abstract

PURPOSE:

Pathogenic leptospires colonize the renal tubules of reservoir hosts of infection and are excreted via urine into the environment. Asymptomatic reservoir hosts include a wide range of domestic and wild animal species and include cattle, dogs, and rats that can persistently excrete large numbers of pathogenic leptospires over many months. A similar presentation has been observed in humans categorized as "long-term asymptomatic individuals" as they excreted leptospires in the absence of any clinical symptoms or positive serology.

EXPERIMENTAL DESIGN:

In the current study, the urine of experimentally infected rats, which showed no clinical signs or positive serology, was analyzed by CE-MS to identify urinary biomarkers of chronic infection.

RESULTS:

A discriminating peptide pattern of 43 polypeptides provided a sensitivity of 93%, a specificity of 83%, and an accuracy of 90% for the identification of urine from chronically infected rats (p < 0.05, AUC > 90%). The majority of discriminating peptides were decreased in abundance in urine of chronically infected rats, including a peptide derived from neprilysin, a membrane metalloendopeptidase, the expression of which has previously been shown to be diminished in infected urine.

CONCLUSION AND CLINICAL RELEVANCE:

Results highlight the diagnostic capabilities of urinary biomarkers to identify reservoir hosts of leptospirosis using CE coupled to MS.

KEYWORDS:

Biomarker; Capillary electrophoresis-mass spectrometry; Leptospira; Leptospirosis; Urine

PMID:
25736478
DOI:
10.1002/prca.201400205
[Indexed for MEDLINE]

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