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Cytokine. 2015 May;73(1):66-73. doi: 10.1016/j.cyto.2015.01.027. Epub 2015 Feb 28.

The potential protective role of the combination of IL-22 and TNF-α against genital tract Chlamydia trachomatis infection.

Author information

1
Department of Obstetrics and Gynecology, Taizhou First People's Hospital, Taizhou, Zhejiang 318020, PR China.
2
First Affiliated Hospital, Wengzhou Medical University, Wengzhou, Zhejiang 325035, PR China.
3
Department of Microbiology and Immunology, Wengzhou Medical University, Wengzhou, Zhejiang 325035, PR China.
4
Department of Microbiology and Immunology, Wengzhou Medical University, Wengzhou, Zhejiang 325035, PR China. Electronic address: wenxu.cn@gmail.com.

Abstract

Th22 cells are a novel class of lymphocytes characterized by the secretion of both IL-22 and TNF-α. In summary, Th22 cells have little or no direct impact on other immune cells, but exert selective effects on epithelia. It is not known, however, whether Th22 cells play a role in genital mucosal immunity. Here, we demonstrate that IL-22 and TNF-α synergistically induce several immunomodulatory molecules, such as the antimicrobial peptide mBD-2 (murine β-defensin 2) and the antimicrobial chemokines CXCL-9, -10, and -11 in primary murine oviduct epithelial cells (MOECs). The induction of innate immunity is relevant in an in vitro infection model, in which MOECs stimulated with Th22 cell supernatants or recombinant IL-22 and TNF-α effectively inhibit the growth of Chlamydia trachomatis and maintain the survival of the epithelia compared with IL-22 or TNF-α alone. In summary, we demonstrate that the Th22 cell cytokines IL-22 and TNF-α play important roles in genital tract infection. The potential for Th22 cell cytokines to modulate innate immune mediators may lead to the development of new topical agents to treat and/or prevent immune-mediated sexually transmitted diseases (STDs). In summary, we demonstrate that IL-22 and TNF-α represent a potent, synergistic cytokine combination for inducing genital mucosal immunity.

KEYWORDS:

Chemokine; Defensin; IL-22; Oviduct epithelial cell; Th22 cell

PMID:
25734538
DOI:
10.1016/j.cyto.2015.01.027
[Indexed for MEDLINE]

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