Send to

Choose Destination
Stem Cells Int. 2015;2015:592090. doi: 10.1155/2015/592090. Epub 2015 Feb 3.

Characterization of human knee and chin adipose-derived stromal cells.

Author information

iBV, UMR CNRS/INSERM, Faculté de Médecine, Université Nice Sophia Antipolis, 06107 Nice Cedex 2, France.
Research, Galderma, Sophia Antipolis, 06410 Biot, France.
iBV, UMR CNRS/INSERM, Faculté de Médecine, Université Nice Sophia Antipolis, 06107 Nice Cedex 2, France ; Plastic, Reconstructive and Hand Surgery Department, Saint Roch Hospital, 06000 Nice, France.


Animal study findings have revealed that individual fat depots are not functionally equivalent and have different embryonic origins depending on the anatomic location. Mouse bone regeneration studies have also shown that it is essential to match the Hox code of transplanted cells and host tissues to achieve correct repair. However, subcutaneous fat depots from any donor site are often used in autologous fat grafting. Our study was thus carried out to determine the embryonic origins of human facial (chin) and limb (knee) fat depots and whether they had similar features and molecular matching patterns. Paired chin and knee fat depots were harvested from 11 subjects and gene expression profiles were determined by DNA microarray analyses. Adipose-derived stromal cells (ASCs) from both sites were isolated and analyzed for their capacity to proliferate, form clones, and differentiate. Chin and knee fat depots expressed a different HOX code and could have different embryonic origins. ASCs displayed a different phenotype, with chin-ASCs having the potential to differentiate into brown-like adipocytes, whereas knee-ASCs differentiated into white adipocytes. These results highlighted different features for these two fat sites and indicated that donor site selection might be an important factor to be considered when applying adipose tissue in cell-based therapies.

Supplemental Content

Full text links

Icon for Hindawi Limited Icon for PubMed Central
Loading ...
Support Center