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Nat Methods. 2015 Apr;12(4):343-6. doi: 10.1038/nmeth.3311. Epub 2015 Mar 2.

High-throughput RNA profiling via up-front sample parallelization.

Author information

1
Department of Therapeutic Radiology, Yale University School of Medicine, New Haven, Connecticut, USA.

Abstract

We describe a method called modular, early-tagged amplification (META) RNA profiling that can quantify a broad panel of microRNAs or mRNAs simultaneously across many samples and requires far less sequence depth than existing digital profiling technologies. The method assigns quantitative tags during reverse transcription to permit up-front sample pooling before competitive amplification and deep sequencing. This simple, scalable and inexpensive approach improves the practicality of large-scale gene expression studies.

PMID:
25730493
PMCID:
PMC4451056
DOI:
10.1038/nmeth.3311
[Indexed for MEDLINE]
Free PMC Article

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