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Nat Methods. 2015 Apr;12(4):323-5. doi: 10.1038/nmeth.3313. Epub 2015 Mar 2.

Simultaneous generation of many RNA-seq libraries in a single reaction.

Author information

1
Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, California, USA.
2
Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA.
3
Bioinformatics Core, University of Massachusetts Medical School, Worcester, Massachusetts, USA.
4
1] Broad Institute of MIT and Harvard, Cambridge, Massachusetts, USA. [2] Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.
5
1] Bioinformatics Core, University of Massachusetts Medical School, Worcester, Massachusetts, USA. [2] Program in Bioinformatics and Integrative Biology, University of Massachusetts Medical School, Worcester, Massachusetts, USA.

Abstract

Although RNA-seq is a powerful tool, the considerable time and cost associated with library construction has limited its utilization for various applications. RNAtag-Seq, an approach to generate multiple RNA-seq libraries in a single reaction, lowers time and cost per sample, and it produces data on prokaryotic and eukaryotic samples that are comparable to those generated by traditional strand-specific RNA-seq approaches.

PMID:
25730492
PMCID:
PMC4712044
DOI:
10.1038/nmeth.3313
[Indexed for MEDLINE]
Free PMC Article

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