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Metallomics. 2015 May;7(5):786-94. doi: 10.1039/c5mt00011d. Epub 2015 Mar 2.

Copper intoxication inhibits aerobic nucleotide synthesis in Streptococcus pneumoniae.

Author information

1
Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, TN 38105-3678, USA. jason.rosch@stjude.org.

Abstract

Copper is universally toxic in excess, a feature exploited by the human immune system to facilitate bacterial clearance. The mechanism of copper intoxication remains unknown for many bacterial species. Here, we demonstrate that copper toxicity in Streptococcus pneumoniae is independent from oxidative stress but, rather, is the result of copper inhibiting the aerobic dNTP biosynthetic pathway. Furthermore, we show that copper-intoxicated S. pneumoniae is rescued by manganese, which is an essential metal in the aerobic nucleotide synthesis pathway. These data provide insight into new targets to enhance copper-mediated toxicity during bacterial clearance.

PMID:
25730343
PMCID:
PMC4431907
DOI:
10.1039/c5mt00011d
[Indexed for MEDLINE]
Free PMC Article

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