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Nat Immunol. 2015 Apr;16(4):426-33. doi: 10.1038/ni.3124. Epub 2015 Mar 2.

A lectin S-domain receptor kinase mediates lipopolysaccharide sensing in Arabidopsis thaliana.

Author information

1
1] Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, Halle, Germany. [2] Phytopathology, TUM School of Life Sciences Weihenstephan, Technische Universität München, Freising-Weihenstephan, Germany.
2
Division of Immunochemistry/Bioanalytical Chemistry, Priority Area Infections, Research Center Borstel, Leibniz-Center for Medicine and Biosciences, Borstel, Germany.
3
Phytopathology, TUM School of Life Sciences Weihenstephan, Technische Universität München, Freising-Weihenstephan, Germany.
4
Stress and Developmental Biology, Leibniz Institute of Plant Biochemistry, Halle, Germany.
5
N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia.

Abstract

The sensing of microbe-associated molecular patterns (MAMPs) triggers innate immunity in animals and plants. Lipopolysaccharide (LPS) from Gram-negative bacteria is a potent MAMP for mammals, with the lipid A moiety activating proinflammatory responses via Toll-like receptor 4 (TLR4). Here we found that the plant Arabidopsis thaliana specifically sensed LPS of Pseudomonas and Xanthomonas. We isolated LPS-insensitive mutants defective in the bulb-type lectin S-domain-1 receptor-like kinase LORE (SD1-29), which were hypersusceptible to infection with Pseudomonas syringae. Targeted chemical degradation of LPS from Pseudomonas species suggested that LORE detected mainly the lipid A moiety of LPS. LORE conferred sensitivity to LPS onto tobacco after transient expression, which demonstrated a key function in LPS sensing and indicated the possibility of engineering resistance to bacteria in crop species.

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PMID:
25729922
DOI:
10.1038/ni.3124
[Indexed for MEDLINE]

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