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Korean J Physiol Pharmacol. 2015 Mar;19(2):119-24. doi: 10.4196/kjpp.2015.19.2.119. Epub 2015 Feb 25.

Effects on g2/m phase cell cycle distribution and aneuploidy formation of exposure to a 60 Hz electromagnetic field in combination with ionizing radiation or hydrogen peroxide in l132 nontumorigenic human lung epithelial cells.

Author information

  • 1Graduate School of Pharmaceutical Sciences, Ewha Womans University, Seoul 120-750, Korea.
  • 2Department of Biomedical Sciences, College of Medicine, Inha University, Incheon 402-751, Korea.
  • 3Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 580-185, Korea.
  • 4Power Systems Research Division, Electrical Environment Team, Korea Electrotechnology Research Institute, Changwon 642-120, Korea.

Abstract

The aim of the present study was to assess whether exposure to the combination of an extremely low frequency magnetic field (ELF-MF; 60 Hz, 1 mT or 2 mT) with a stress factor, such as ionizing radiation (IR) or H2O2, results in genomic instability in non-tumorigenic human lung epithelial L132 cells. To this end, the percentages of G2/M-arrested cells and aneuploid cells were examined. Exposure to 0.5 Gy IR or 0.05 mM H2O2 for 9 h resulted in the highest levels of aneuploidy; however, no cells were observed in the subG1 phase, which indicated the absence of apoptotic cell death. Exposure to an ELF-MF alone (1 mT or 2 mT) did not affect the percentages of G2/M-arrested cells, aneuploid cells, or the populations of cells in the subG1 phase. Moreover, when cells were exposed to a 1 mT or 2 mT ELF-MF in combination with IR (0.5 Gy) or H2O2 (0.05 mM), the ELF-MF did not further increase the percentages of G2/M-arrested cells or aneuploid cells. These results suggest that ELF-MFs alone do not induce either G2/M arrest or aneuploidy, even when administered in combination with different stressors.

KEYWORDS:

Aneuploidy; Extremely low frequency; G2/M phase; H2O2; Ionizing radiation

PMID:
25729273
PMCID:
PMC4342731
DOI:
10.4196/kjpp.2015.19.2.119
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