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J Mol Biol. 2015 Jul 31;427(15):2414-2417. doi: 10.1016/j.jmb.2015.02.018. Epub 2015 Feb 26.

Exon Skipping Is Correlated with Exon Circularization.

Author information

1
Department of Plant Sciences, University of Oxford, South Parks Road, OX1 3RB Oxford, UK. Electronic address: steven.kelly@plants.ox.ac.uk.
2
The Genome Analysis Centre, Norwich Research Park, NR4 7UH Norwich, UK.
3
Sir William Dunn of Pathology, University of Oxford, South Parks Road, OX1 3RE Oxford, UK.
4
Sir William Dunn of Pathology, University of Oxford, South Parks Road, OX1 3RE Oxford, UK; Center for Molecular Medicine, University of Cologne, Robert-Koch-Straße 21, D-50931 Cologne, Germany. Electronic address: argyris.papantonis@uni-koeln.de.

Abstract

Circular RNAs are found in a wide range of organisms and it has been proposed that they perform disparate functions. However, how RNA circularization is connected to alternative splicing remains largely unexplored. Here, we stimulated primary human endothelial cells with tumor necrosis factor α or tumor growth factor β, purified RNA, generated >2.4 billion RNA-seq reads, and used a custom pipeline to characterize circular RNAs derived from coding exons. We find that circularization of exons is widespread and correlates with exon skipping, a feature that adds considerably to the regulatory complexity of the human transcriptome.

KEYWORDS:

alternative splicing; circRNA; cotranscriptional; turnover

Comment in

PMID:
25728652
DOI:
10.1016/j.jmb.2015.02.018
[Indexed for MEDLINE]

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