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FEBS Lett. 2015 Apr 2;589(8):924-32. doi: 10.1016/j.febslet.2015.02.022. Epub 2015 Feb 26.

The histone deacetylase Rpd3/Sin3/Ume6 complex represses an acetate-inducible isoform of VTH2 in fermenting budding yeast cells.

Author information

1
Inserm U1085 IRSET, Université de Rennes 1, 35042 Rennes, France.
2
Rowan University, School of Osteopathic Medicine, Stratford, NJ 08084, USA.
3
Inserm U1085 IRSET, Université de Rennes 1, 35042 Rennes, France. Electronic address: michael.primig@inserm.fr.

Abstract

The tripartite Rpd3/Sin3/Ume6 complex represses meiotic isoforms during mitosis. We asked if it also controls starvation-induced isoforms. We report that VTH1/VTH2 encode acetate-inducible isoforms with extended 5'-regions overlapping antisense long non-coding RNAs. Rpd3 and Ume6 repress the long isoform of VTH2 during fermentation. Cells metabolising glucose contain Vth2, while the protein is undetectable in acetate and during sporulation. VTH2 is a useful model locus to study mechanisms implicating promoter directionality, lncRNA transcription and post-transcriptional control of gene expression via 5'-UTRs. Since mammalian genes encode transcript isoforms and Rpd3 is conserved, our findings are relevant for gene expression in higher eukaryotes.

KEYWORDS:

5′-UTR; Isoform; RPD3; UME6; VTH1; VTH2

PMID:
25728275
DOI:
10.1016/j.febslet.2015.02.022
[Indexed for MEDLINE]
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