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J Clin Virol. 2015 Mar;64:88-91. doi: 10.1016/j.jcv.2015.01.008. Epub 2015 Jan 13.

Evaluation of human papillomavirus DNA detection in samples obtained for routine Chlamydia trachomatis screening.

Author information

1
Division of Laboratory Medicine, Department of Clinical Microbiology, Jan Waldenströms gata 59, 20502 Malmö, Sweden.
2
Department of Dermatovenereology, Karolinska University Hospital Solna, 171 76 Stockholm, Sweden.
3
Department of Laboratory Medicine, Karolinska Institutet, F56Huddinge, 14188 Stockholm, Sweden. Electronic address: joakim.dillner@ki.se.

Abstract

BACKGROUND:

The costs and logistics involved in obtaining samples is a bottleneck in large-scale studies of the circulation of human papillomavirus (HPV), which are useful for monitoring and optimisation of HPV-vaccination programs. Residual samples obtained after screening for Chlamydia trachomatis could constitute a convenient, low-cost solution.

OBJECTIVES:

We evaluated HPV DNA detection and typing using (i) the residual samples routinely taken for C. trachomatis screening or (ii) the sample types used in large-scale phase III HPV vaccination trials (cervical, vulvar, labial, perineal, perianal, scrotal and penile shaft samples).

STUDY DESIGN:

Samples from 127 men and 110 women attending two sexual health clinics were analysed using PCR for HPV DNA, with typing using mass spectrometry.

RESULTS:

The HPV DNA prevalence was 7.1% in male urine samples, but 57.3% in female urine/vaginal samples, which was even higher than the HPV prevalence found in cervical samples (54.1%). The sensitivity for HPV DNA detection in the urine/vaginal samples was 7.9% (95% CI 3.0-16.4) for men and 78.9% (95% CI 67.6-87.7) for women, using detection in any one of the reference samples as reference. With cervical samples as reference, the sensitivity was 89.3 % (95% CI 78.1-95.9).

CONCLUSIONS:

Among men, low sensitivity of urine for HPV detection suggests limited usefulness. Among women, the high sensitivity of urine/vaginal samples for HPV detection suggests a useful low-cost solution for the study of HPV epidemiology.

KEYWORDS:

Human papillomavirus DNA detection; Human papillomavirus prevalence; Sampling technique evaluation

PMID:
25728085
DOI:
10.1016/j.jcv.2015.01.008
[Indexed for MEDLINE]

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