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Curr Protoc Mouse Biol. 2015 Mar 2;5(1):65-84. doi: 10.1002/9780470942390.mo140111.

Glucose tolerance tests for systematic screening of glucose homeostasis in mice.

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Institute of Experimental Genetics, German Mouse Clinic, Helmholtz-Zentrum München, German Center for Environmental Health, Neuherberg, Germany; German Research Center for Diabetes Research, Neuherberg, Germany; These authors contributed equally to this work.


This article presents a detailed description of intraperitoneal and oral glucose tolerance tests in mice. The former is widely used in initial high-throughput phenotyping of mutant mice to assess a diabetic phenotype and alterations in glucose homeostasis. Each protocol provides a comprehensive description of each step in the workflow, including variation of the standard protocol under particular circumstances (e.g., sensitivity to food deprivation, excessive deviations in body composition, or need for extra blood samples for additional analyses). We also describe how reduction of body mass and body temperature can be used as additional readouts to monitor metabolic function in response to food deprivation.


IPGTT; OGTT; glucose intolerance; glucose tolerance test; high-throughput phenotyping; mouse models; thermovision; type 2 diabetes mellitus

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