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Mutat Res Genet Toxicol Environ Mutagen. 2015 Jan 1;777:68-72. doi: 10.1016/j.mrgentox.2014.12.002. Epub 2014 Dec 15.

Ames positive boronic acids are not all eukaryotic genotoxins.

Author information

  • 1Gentronix Ltd., BioHub at Alderley Park, Cheshire, SK10 4TG, United Kingdom.
  • 2Gentronix Ltd., BioHub at Alderley Park, Cheshire, SK10 4TG, United Kingdom; Faculty of Life Sciences, University of Manchester, M13 9PL, United Kingdom. Electronic address: richard.walmsley@manchester.ac.uk.

Abstract

Boronic acids and their derivatives have been exploited for their pharmacological activity and their utility as intermediates in the synthesis of novel non-boron containing compounds. A recent study reported that boronic acids are bacterial mutagens. Here, results are reported from the testing of nine boronic acids using the pan-mechanistic eukaryotic GADD45a genotoxicity assays, BlueScreen HC and GreenScreen HC. Positive results were produced for one compound in GreenScreen and four compounds in BlueScreen. Only negative results were produced when tested with S9 metabolic activation. These data suggest that there is not a general genotoxic liability in eukaryotes, within this chemical domain. Furthermore, they are not potent eukaryotic genotoxins: positive results were produced only at concentrations between 1mM and 10mM. Their presence as low concentration contaminants or impurities would be unlikely to produce misleading positive results for a test material.

KEYWORDS:

Ames; BlueScreen HC; Boronic acids; Eukaryotic genotoxins; GADD45a; GreenScreen HC

PMID:
25726177
DOI:
10.1016/j.mrgentox.2014.12.002
[PubMed - indexed for MEDLINE]
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