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Mutat Res Genet Toxicol Environ Mutagen. 2015 Jan 1;777:29-32. doi: 10.1016/j.mrgentox.2014.11.005. Epub 2014 Nov 27.

Forskolin: genotoxicity assessment in Allium cepa.

Author information

1
Department of Genetics, Osmania University, Hyderabad, Andhra Pradesh, India.
2
Department of Genetics, Osmania University, Hyderabad, Andhra Pradesh, India. Electronic address: drparveenjahan@osmania.ac.in.

Abstract

Forskolin, a diterpene, 7β-acetoxy-8,13-epoxy-1α,6β,9α-trihydroxy-labd-14-en-11-one (C22H34O7) isolated from Coleus forskohlii, exerts multiple physiological effects by stimulating the enzyme adenylate cyclase and increasing cyclic adenosine monophosphate (cAMP) concentrations. Forskolin is used in the treatment of hypertension, congestive heart failure, eczema, and other diseases. A cytogenetic assay was performed in Allium cepa to assess possible genotoxic effects of forskolin. Forskolin was tested at concentrations 5-100 μM for exposure periods of 24 or 48 h. Treated samples showed significant reductions in mitotic index (p < 0.05) and increases in the frequency of chromosome aberrations (p < 0.01) at both exposure times. The treated meristems showed chromosome aberrations including sticky metaphases, sticky anaphases, laggard, anaphase bridges, micronuclei, polyploidy, fragments, breaks, and C-mitosis. Forskolin may cause genotoxic effects and further toxicological evaluations should be conducted to ensure its safety.

KEYWORDS:

Allium cepa; Chromosomal aberrations; Forskolin; Genotoxiciy; Mitotic index

PMID:
25726172
DOI:
10.1016/j.mrgentox.2014.11.005
[Indexed for MEDLINE]

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