Format

Send to

Choose Destination
J Cyst Fibros. 2015 Jul;14(4):468-73. doi: 10.1016/j.jcf.2015.02.004. Epub 2015 Feb 26.

Impact of storage conditions on metabolite profiles of sputum samples from persons with cystic fibrosis.

Author information

1
Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI 48109, United States.
2
Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI 48109, United States.
3
Department of Pediatrics and Communicable Diseases, University of Michigan Medical School, Ann Arbor, MI 48109, United States. Electronic address: jlipuma@med.umich.edu.

Abstract

BACKGROUND:

Although recent studies have begun to elucidate how airway microbial community structure relates to lung disease in cystic fibrosis (CF), microbial community activity and the host's response to changes in this activity are poorly understood. Metabolomic profiling provides a means to investigate microbial activity and human cell activity within diseased airways. However, variables in sample storage and shipping likely affect downstream analyses and standards for sample handling are lacking.

METHODS:

We assessed the impact of sample storage conditions on liquid chromatography mass spectrometry analysis of CF sputum samples.

RESULTS:

Significant changes in global metabolomic profiles occurred in samples stored at room temperature or at 4°C for longer than one day. Untargeted metabolomic profiles were stable in sputum samples stored at -20°C or -80°C for at least 28 days. Quorum sensing molecules and phenazines, both considered important to the in vivo activity of Pseudomonas during airway infection, were detected after sample storage at room temperature for five days.

CONCLUSIONS:

Sputum samples can be stored at -20°C or -80°C for weeks with minimal effect on global metabolomic profiles. This observation provides guidance in designing metabolomic studies that have the potential to deepen our understanding of how airway microbial communities impact lung disease progression in CF.

KEYWORDS:

Cystic fibrosis; Metabolome; Sputum

PMID:
25725986
PMCID:
PMC4947390
DOI:
10.1016/j.jcf.2015.02.004
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center