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Biomaterials. 2015 May;49:125-34. doi: 10.1016/j.biomaterials.2015.01.042. Epub 2015 Feb 16.

Tailoring the stealth properties of biocompatible polysaccharide nanocontainers.

Author information

1
Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany.
2
Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany; Department of Hematology, Medical Oncology, and Pneumology, University Medical, Center Mainz, Langenbeckstr. 1, 55131 Mainz, Germany.
3
Institute of Cell Biology and Immunology, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany.
4
Department of Hematology, Medical Oncology, and Pneumology, University Medical, Center Mainz, Langenbeckstr. 1, 55131 Mainz, Germany.
5
Biontech AG, An der Goldgrube 12, 55131 Mainz, Germany; TRON - Translational Oncology at the University Medical Center of Johannes Gutenberg University Mainz, Langenbeckstr 1, 55131 Mainz, Germany.
6
Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany. Electronic address: wurm@mpip-mainz.mpg.de.

Abstract

Fundamental development of a biocompatible and degradable nanocarrier platform based on hydroxyethyl starch (HES) is reported. HES is a derivative of starch and possesses both high biocompatibility and improved stability against enzymatic degradation; it is used to prepare nanocapsules via the polyaddition reaction at the interface of water nanodroplets dispersed in an organic miniemulsion. The synthesized hollow nanocapsules can be loaded with hydrophilic guests in its aqueous core, tuned in size, chemically functionalized in various pathways, and show high shelf life stability. The surface of the HES nanocapsules is further functionalized with poly(ethylene glycol) via different chemistries, which substantially enhanced blood half-life time. Importantly, methods for precise and reliable quantification of the degree of functionalization are also introduced, which enable the precise control of the chemistry on the capsules' surface. The stealth properties of these capsules is studied both in-vitro and in-vivo. The functionalized nanocapsules serve as a modular platform for specific cell targeting, as they show no unspecific up-taken by different cell types and show very long circulating time in blood (up to 72 h).

KEYWORDS:

Hydroxyl ethyl starch; Miniemulsion; Nanocarrier; PEGylation; Stealth effect

[Indexed for MEDLINE]

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