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J Reprod Immunol. 2015 Aug;110:89-101. doi: 10.1016/j.jri.2015.01.006. Epub 2015 Feb 12.

Characterization of immune cells and cytokine localization in the rat utero-placental unit mid- to late gestation.

Author information

1
Department of Biology, University of Ottawa, Ottawa, Ontario, Canada K1N 6N5. Electronic address: dtess036@uottawa.ca.
2
Department of Pediatrics and the Graduate Program in Medical Sciences, McMaster University, Hamilton, Ontario, Canada L8N 3Z5.
3
Department of Obstetrics and Gynecology, McMaster University, Hamilton, Ontario, Canada L8N 3Z5.
4
Chronic Disease Program, The Ottawa Hospital Research Institute, Ottawa, Ontario, Canada K1Y 4E9.
5
Department of Biomedical and Molecular Sciences, Queens University, Kingston, Ontario, Canada K7L 3N6.
6
Chronic Disease Program, The Ottawa Hospital Research Institute, Ottawa, Ontario, Canada K1Y 4E9; Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, The Ottawa Hospital, University of Ottawa, Ottawa, Ontario, Canada K1H 8L6.

Abstract

The success of pregnancy is dependent on the precise regulation of the immune response within the utero-placental environment. Rats are beginning to be widely used as a model for human immune-related pregnancy complications. However, our knowledge of immune cells and cytokine localization in the rat utero-placental tissue is limited. The current study aimed to localize the immune cell populations, including uterine natural killer (uNK) cells, neutrophils, and macrophages within the rat utero-placental unit at two crucial gestational ages, gestational days 15.5 and 18.5. In addition, we characterized the distribution of the cytokines TNFα, IFNγ, and IL-10 in the utero-placental regions at both the above-mentioned gestational ages. Our study has demonstrated co-localization TNFα and IFNγ with uNK cells in perivascular regions of the rat mesometrial triangle at both gestational ages. Neutrophils and IL-10-positive cells were localized at the maternal-fetal interface and in the spiral artery lumen of the rat mesometrial triangle at both gestational ages. TNFα and IL-10 demonstrated a temporal change in the localization from GD15.5 to GD18.5, which coincides with the leading edge of trophoblast invasion into the mesometrial triangle. The current study furthers our knowledge of the localization of uterine immune cells and relevant cytokines, and provides a base from which to research the function of these immune cells and cytokines during rat pregnancy as a model to study human immune-related pregnancy complications.

KEYWORDS:

Interferon gamma; Interleukin 10; Neutrophils; Rat pregnancy; Tumor necrosis factor alpha; Uterine natural killer cells

PMID:
25725501
DOI:
10.1016/j.jri.2015.01.006
[Indexed for MEDLINE]

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