Format

Send to

Choose Destination
FEMS Microbiol Lett. 2015 Apr;362(7). pii: fnv029. doi: 10.1093/femsle/fnv029. Epub 2015 Feb 26.

Gene mdpC plays a regulatory role in the methyl-tert-butyl ether degradation pathway of Methylibium petroleiphilum strain PM1.

Author information

1
Department of Land, Air and Water Resources, University of California, Davis, CA 95616, USA.
2
Department of Environmental Toxicology, University of California, Davis, CA 95616, USA.
3
Department of Land, Air and Water Resources, University of California, Davis, CA 95616, USA Biological Sciences Department, Marquette University, Milwaukee, WI 53201, USA krassimira.hristova@marquette.edu.

Abstract

Among the few bacteria known to utilize methyl tert-butyl ether (MTBE) as a sole carbon source, Methylibium petroleiphilum PM1 is a well-characterized organism with a sequenced genome; however, knowledge of the genetic regulation of its MTBE degradation pathway is limited. We investigated the role of a putative transcriptional activator gene, mdpC, in the induction of MTBE-degradation genes mdpA (encoding MTBE monooxygenase) and mdpJ (encoding tert-butyl alcohol hydroxylase) of strain PM1 in a gene-knockout mutant mdpC(-). We also utilized quantitative reverse transcriptase PCR assays targeting genes mdpA, mdpJ and mdpC to determine the effects of the mutation on transcription of these genes. Our results indicate that gene mdpC is involved in the induction of both mdpA and mdpJ in response to MTBE and tert-butyl alcohol (TBA) exposure in PM1. An additional independent mechanism may be involved in the induction of mdpJ in the presence of TBA.

KEYWORDS:

MTBE; MdpC; Methylibium petroleiphilum strain PM1; degradation pathway; mdpC− mutant; regulation

PMID:
25724531
PMCID:
PMC4459789
DOI:
10.1093/femsle/fnv029
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Silverchair Information Systems Icon for PubMed Central
Loading ...
Support Center