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Mol Imaging Biol. 2015 Oct;17(5):633-42. doi: 10.1007/s11307-015-0834-8.

Optical Imaging of Tumor Response to Hyperbaric Oxygen Treatment and Irradiation in an Orthotopic Mouse Model of Head and Neck Squamous Cell Carcinoma.

Author information

  • 1Department of Oral and Maxillofacial Surgery, Erasmus Medical Center, PO Box 2040, 3000 CA, Rotterdam, The Netherlands. j.braks@erasmusmc.nl.
  • 2Department of Oral and Maxillofacial Surgery, Erasmus Medical Center, PO Box 2040, 3000 CA, Rotterdam, The Netherlands.
  • 3Department of Pathology, Erasmus Medical Center, PO Box 2040, 3000 CA, Rotterdam, The Netherlands.
  • 4Department of Genetics, Erasmus Medical Center, PO Box 2040, 3000 CA, Rotterdam, The Netherlands.
  • 5Department of Otorhinolaryngology and Head & Neck Surgery, Erasmus Medical Center, PO Box 1738, 3015 CE, Rotterdam, Netherlands.
  • 6Department of Radiation Oncology, Erasmus Medical Center, PO Box 2040, 3000 CA, Rotterdam, The Netherlands.
  • 7Department of Genetics, Erasmus Medical Center, PO Box 2040, 3000 CA, Rotterdam, The Netherlands. j.essers@erasmusmc.nl.
  • 8Department of Radiation Oncology, Erasmus Medical Center, PO Box 2040, 3000 CA, Rotterdam, The Netherlands. j.essers@erasmusmc.nl.
  • 9Department of Vascular Surgery, Erasmus Medical Center, PO Box 2040, 3000 CA, Rotterdam, The Netherlands. j.essers@erasmusmc.nl.

Abstract

PURPOSE:

Hyperbaric oxygen therapy (HBOT) is used in the treatment of radiation-induced tissue injury but its effect on (residual) tumor tissue is indistinct and therefore investigated in this study.

PROCEDURES:

Orthotopic FaDu tumors were established in mice, and the response of the (irradiated) tumors to HBOT was monitored by bioluminescence imaging. Near infrared fluorescence imaging using AngioSense750 and Hypoxisense680 was applied to detect tumor vascular permeability and hypoxia.

RESULTS:

HBOT treatment resulted in accelerated growth of non-irradiated tumors, but mouse survival was improved. Tumor vascular leakiness and hypoxia were enhanced after HBOT, whereas histological characteristics, epithelial-to-mesenchymal transition markers, and metastatic incidence were not influenced.

CONCLUSIONS:

Squamous cell carcinoma responds to HBOT with respect to tumor growth, vascular permeability, and hypoxia, which may have implications for its use in cancer patients. The ability to longitudinally analyze tumor characteristics highlights the versatility and potential of optical imaging methods in oncological research.

KEYWORDS:

Animal model; Head and neck cancer; Hyperbaric oxygen therapy; Lymph node metastasis; Near infrared fluorescence; Optical imaging; Radiation; Squamous cell carcinoma

PMID:
25724406
PMCID:
PMC4768231
DOI:
10.1007/s11307-015-0834-8
[PubMed - indexed for MEDLINE]
Free PMC Article
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