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Curr Opin Struct Biol. 2015 Feb;30:100-111. doi: 10.1016/j.sbi.2015.02.002. Epub 2015 Feb 24.

The structural biology of CRISPR-Cas systems.

Author information

1
Department of Molecular & Cell Biology, University of California, Berkeley, CA 94720, USA.
2
Department of Molecular & Cell Biology, University of California, Berkeley, CA 94720, USA; Department of Chemistry, University of California, Berkeley, CA 94720, USA; Physical Biosciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA 94720, USA; Howard Hughes Medical Institute (HHMI), University of California, Berkeley, CA 94720, USA. Electronic address: doudna@berkeley.edu.

Abstract

Prokaryotic CRISPR-Cas genomic loci encode RNA-mediated adaptive immune systems that bear some functional similarities with eukaryotic RNA interference. Acquired and heritable immunity against bacteriophage and plasmids begins with integration of ∼30 base pair foreign DNA sequences into the host genome. CRISPR-derived transcripts assemble with CRISPR-associated (Cas) proteins to target complementary nucleic acids for degradation. Here we review recent advances in the structural biology of these targeting complexes, with a focus on structural studies of the multisubunit Type I CRISPR RNA-guided surveillance and the Cas9 DNA endonuclease found in Type II CRISPR-Cas systems. These complexes have distinct structures that are each capable of site-specific double-stranded DNA binding and local helix unwinding.

PMID:
25723899
PMCID:
PMC4417044
DOI:
10.1016/j.sbi.2015.02.002
[Indexed for MEDLINE]
Free PMC Article

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