Format

Send to

Choose Destination
Saudi Med J. 2015 Feb;36(2):248-51. doi: 10.15537/smj.2015.2.10874.

Molecular investigation of mixed malaria infections in southwest Saudi Arabia.

Author information

1
Department of Biology, Faculty of Science, King Khalid University, PO Box 9004, Abha, Kingdom of Saudi Arabia. Tel. +966 (17) 544804040. Fax. +966 (17) 2417851. E-mail. saad1426@gmail.com.

Abstract

OBJECTIVE:

To investigate the incidence of mixed-species (MS) malaria infection, and compare the results with microscopically confirmed cases of malaria.

METHODS:

During 2010, blood spots collected from 371 clinically suspected cases of malaria were microscopically examined in a cross-sectional study. The DNA was extracted from the samples, and a nested polymerase chain reaction (PCR) was performed. The results obtained by the 2 methods were compared.

RESULTS:

From the microscopic analysis it was determined that 369 samples (99.5%) were positive for Plasmodium falciparum (P. falciparum) and 2 were Plasmodium vivax (P. vivax) mono-infections. There were no mixed malaria infections. The PCR analysis, however, showed that in 7 cases (1.9%) the infection was caused by MS malaria comprising of P. falciparum and P. vivax, 2 of these representing the cases that were microscopically diagnosed as P. vivax mono-infections. All cases were negative for Plasmodium malariae, Plasmodium ovale, and Plasmodium knowlesi.

CONCLUSION:

Mixed malaria infections are currently overlooked when using microscopy. The PCR assays are essential complementary techniques that should be used with microscopic examination of blood smears.

PMID:
25719595
PMCID:
PMC4375708
DOI:
10.15537/smj.2015.2.10874
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Saudi Medical Journal Icon for PubMed Central
Loading ...
Support Center