Abstract
Here we showed that exogenous miR-372 expression and knockdown of p62 (sequestosome1 or SQSTM1), both increased migration of head and neck squamous cell carcinoma (HNSCC) cells. p62 induced phase II detoxification enzyme NADPH quinone oxidoreductase 1 (NQO1), which decreased ROS levels and cell migration. Also, miR-372 decreased p62 during hypoxia, thus increasing cell migration. Levels of miR-372 and p62 inversely correlated in human HNSCC tissues. Plasma levels of miR-372 was associated with advanced tumor stage and patient mortality. Both plasma and salivary miR-372 levels were decreased after tumor resection. We conclude that miR-372 decreases p62, thus increasing ROS and motility in HNSCC cells.
Keywords:
carcinoma; hypoxia; invasion; miR-372; mouth.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Carcinoma, Squamous Cell / genetics
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Carcinoma, Squamous Cell / metabolism
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Carcinoma, Squamous Cell / pathology
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Carcinoma, Squamous Cell / therapy*
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Cell Hypoxia / genetics
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Cell Line, Tumor
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Cell Movement / genetics
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HEK293 Cells
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Head and Neck Neoplasms / genetics
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Head and Neck Neoplasms / metabolism
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Head and Neck Neoplasms / pathology
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Head and Neck Neoplasms / therapy*
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Humans
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Mice
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Mice, Nude
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MicroRNAs / administration & dosage*
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MicroRNAs / biosynthesis
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MicroRNAs / genetics
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NAD(P)H Dehydrogenase (Quinone) / biosynthesis
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Prognosis
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RNA-Binding Proteins / antagonists & inhibitors*
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RNA-Binding Proteins / biosynthesis
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Reactive Oxygen Species / metabolism
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Squamous Cell Carcinoma of Head and Neck
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Xenograft Model Antitumor Assays
Substances
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MIRN372 microRNA, human
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MicroRNAs
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P62 protein, human
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RNA-Binding Proteins
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Reactive Oxygen Species
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NAD(P)H Dehydrogenase (Quinone)
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NQO1 protein, human