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Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):E1057-66. doi: 10.1073/pnas.1421202112. Epub 2015 Feb 23.

On the principle of ion selectivity in Na+/H+-coupled membrane proteins: experimental and theoretical studies of an ATP synthase rotor.

Author information

1
Theoretical Molecular Biophysics Section, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892; Theoretical Molecular Biophysics Group and jose.faraldo@nih.gov vanessa.leonealvarez@nih.gov.
2
Department of Structural Biology, Max Planck Institute of Biophysics, 60438 Frankfurt am Main, Germany; and Cluster of Excellence Macromolecular Complexes, Goethe University, 60438 Frankfurt am Main, Germany.

Abstract

Numerous membrane transporters and enzymes couple their mechanisms to the permeation of Na(+) or H(+), thereby harnessing the energy stored in the form of transmembrane electrochemical potential gradients to sustain their activities. The molecular and environmental factors that control and modulate the ion specificity of most of these systems are, however, poorly understood. Here, we use isothermal titration calorimetry to determine the Na(+)/H(+) selectivity of the ion-driven membrane rotor of an F-type ATP synthase. Consistent with earlier theoretical predictions, we find that this rotor is significantly H(+) selective, although not sufficiently to be functionally coupled to H(+), owing to the large excess of Na(+) in physiological settings. The functional Na(+) specificity of this ATP synthase thus results from two opposing factors, namely its inherent chemical selectivity and the relative availability of the coupling ion. Further theoretical studies of this membrane rotor, and of two others with a much stronger and a slightly weaker H(+) selectivity, indicate that, although the inherent selectivity of their ion-binding sites is largely set by the balance of polar and hydrophobic groups flanking a conserved carboxylic side chain, subtle variations in their structure and conformational dynamics, for a similar chemical makeup, can also have a significant contribution. We propose that the principle of ion selectivity outlined here may provide a rationale for the differentiation of Na(+)- and H(+)-coupled systems in other families of membrane transporters and enzymes.

KEYWORDS:

binding thermodynamics; energy transduction; ion-coupled transport; membrane bioenergetics; molecular motor

PMID:
25713346
PMCID:
PMC4364180
DOI:
10.1073/pnas.1421202112
[Indexed for MEDLINE]
Free PMC Article

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