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Mass Spectrom Rev. 2016 Jan-Feb;35(1):3-21. doi: 10.1002/mas.21457. Epub 2015 Feb 20.

Mass spectrometry of extracellular vesicles.

Author information

1
Mass Spectrometry and Proteomics, Institute of Biosciences and BioResources, National Research Council of Italy, Naples, Italy.
2
Division of Nephrology, Department of Cardio-Vascular Sciences, Second University of Naples, Naples, Italy.
3
Mass Spectrometry Proteomics Group, Institute of Organic Chemistry, Research Centre for Natural Sciences of the Hungarian Academy of Sciences, Budapest, Hungary.
4
Department of Genetics, Cell- and Immunobiology, Semmelweis University, Budapest, Hungary.

Abstract

The review briefly summaries main features of extracellular vesicles, a joint terminology for exosomes, microvesicles, and apoptotic vesicles. These vesicles are in the center of interest in biology and medical sciences, and form a very active field of research. Mass spectrometry (MS), with its specificity and sensitivity, has the potential to identify and characterize molecular composition of these vesicles; but as yet there are only a limited, but fast-growing, number of publications that use MS workflows in this field. MS is the major tool to assess protein composition of extracellular vesicles: qualitative and quantitative proteomics approaches are both reviewed. Beside proteins, lipid and metabolite composition of vesicles might also be best assessed by MS techniques; however there are few applications as yet in this respect. The role of alternative analytical approaches, like gel-based proteomics and antibody-based immunoassays, are also mentioned. The objective of the review is to give an overview of this fast-growing field to help orient MS-based research on extracellular vesicles.

KEYWORDS:

biomarker discovery; extracellular vesicles; lipids; proteins; subcellular proteomics

PMID:
25705034
DOI:
10.1002/mas.21457
[Indexed for MEDLINE]

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