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J Voice. 2015 Sep;29(5):560-3. doi: 10.1016/j.jvoice.2014.10.013. Epub 2015 Feb 19.

Effects of Electronic Nicotine Delivery System on Larynx: Experimental Study.

Author information

1
Okmeydanı Training and Research Hospital, ENT Clinic, Istanbul, Turkey. Electronic address: ziyasalturk@gmail.com.
2
Okmeydanı Training and Research Hospital, Pathology Clinic, Istanbul, Turkey.
3
Okmeydanı Training and Research Hospital, ENT Clinic, Istanbul, Turkey.

Abstract

OBJECTIVE:

We aimed to assess the effects of electronic nicotine delivery system (ENDS) or also termed electronic cigarette vapor on the laryngeal mucosa of rats.

MATERIALS AND METHODS:

Sixteen female Wistar albino rats were divided into two groups. The study group was exposed to ENDS vapor for 1 hour/day for 4 weeks. The control group was not subjected to any chemical or physical stimulus. The vocal folds of the study and control group rats were evaluated histopathologically by hematoxylin and eosin staining and immunohistochemically by Ki67 staining. Epithelial distribution, inflammation, hyperplasia, and metaplasia were evaluated.

RESULTS:

Epithelial distribution and inflammation did not differ between the two groups. Two cases of hyperplasia were detected in the study group but there was no hyperplasia in the control group. Four cases of metaplasia were detected in the study group and one case in the control group. Statistical analysis revealed no significant difference between the study and control groups (P = 0.131 and 0.106, respectively).

CONCLUSIONS:

Exposure to ENDS for 4 weeks caused hyperplasia and metaplasia of the laryngeal mucosa of rats but this was not significant statistically. These results implemented that further studies with larger cohort and longer duration are required to evaluate long-term effects.

KEYWORDS:

Electronic cigarette; Electronic nicotine delivery system; Histopathology; Larynx; Metaplasia

PMID:
25704471
DOI:
10.1016/j.jvoice.2014.10.013
[Indexed for MEDLINE]

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