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Int J Biol Macromol. 2015 May;76:242-53. doi: 10.1016/j.ijbiomac.2015.01.060. Epub 2015 Feb 20.

Hericium erinaceus polysaccharide-protein HEG-5 inhibits SGC-7901 cell growth via cell cycle arrest and apoptosis.

Author information

1
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, P.R. China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, P.R. China.
2
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, P.R. China. Electronic address: fengjiecui@163.com.
3
School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, P.R. China.
4
National Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, P.R. China. Electronic address: yangyan@saas.sh.cn.
5
National Engineering Research Center of Edible Fungi, Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, P.R. China.
6
Institute of Quality and Standard for Agroproducts, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, P.R. China. Electronic address: lfping2005@gmail.com.

Abstract

HEG-5 is a novel polysaccharide-protein purified from the fermented mycelia of Hericium erinaceus CZ-2. The present study aims to investigate the effects of HEG-5 on proliferation, cell cycle and apoptosis of human gastric cancer cells SGC-7901. Here, we first uncover that HEG-5 significantly inhibited the proliferation and colony formation of SGC-7901 cells by promoting apoptosis and cell cycle arrest at S phase. RT-PCR and Western blot analysis suggested that HEG-5 could decrease the expressions of Bcl2, PI3K and AKT1, while increase the expressions of Caspase-8, Caspase-3, p53, CDK4, Bax and Bad. These findings indicated that the Caspase-8/-3-dependent, p53-dependent mitochondrial-mediated and PI3k/Akt signaling pathways involved in the molecular events of HEG-5 induced apoptosis and cell cycle arrest. Thus, our study provides in vitro evidence that HEG-5 may be taken as a potential candidate for treating gastric cancer.

KEYWORDS:

Anti-tumor activity; Apoptosis; Cell cycle; Hericium erinaceus; Polysaccharide-protein

PMID:
25703932
DOI:
10.1016/j.ijbiomac.2015.01.060
[Indexed for MEDLINE]

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