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FEBS Lett. 2015 Mar 12;589(6):779-86. doi: 10.1016/j.febslet.2015.02.011. Epub 2015 Feb 18.

Experimental evidence suggesting that H2O2 is produced within the thylakoid membrane in a reaction between plastoquinol and singlet oxygen.

Author information

1
Department of Biochemistry/Molecular Plant Biology, University of Turku, FI-20014 Turku, Finland.
2
Laboratory of Organic Chemistry and Chemical Biology, Department of Chemistry, University of Turku, FI-20014 Turku, Finland.
3
Department of Biochemistry/Molecular Plant Biology, University of Turku, FI-20014 Turku, Finland. Electronic address: esatyy@utu.fi.

Abstract

Plastoquinol (PQH2-9) and plastoquinone (PQ-9) mediate photosynthetic electron transfer. We isolated PQH2-9 from thylakoid membranes, purified it with HPLC, subjected the purified PQH2-9 to singlet oxygen ((1)O2) and analyzed the products. The main reaction of (1)O2 with PQH2-9 in methanol was found to result in formation of PQ-9 and H2O2, and the amount of H2O2 produced was essentially the same as the amount of oxidized PQH2-9. Formation of H2O2 in the reaction between (1)O2 and PQH2-9 may be an important source of H2O2 within the lipophilic thylakoid membrane.

KEYWORDS:

Hydrogen peroxide; Photosystem II; Plastoquinone; Singlet oxygen; Thylakoid membrane

PMID:
25701589
DOI:
10.1016/j.febslet.2015.02.011
[Indexed for MEDLINE]
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