Format

Send to

Choose Destination
Trends Microbiol. 2015 Apr;23(4):225-32. doi: 10.1016/j.tim.2015.01.008. Epub 2015 Feb 17.

Harnessing CRISPR-Cas systems for bacterial genome editing.

Author information

1
Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, Raleigh, NC 27606, USA; Genomic Sciences Graduate Program, North Carolina State University, Raleigh, NC 27695, USA.
2
Department of Food, Bioprocessing, and Nutrition Sciences, North Carolina State University, Raleigh, NC 27606, USA. Electronic address: rbarran@ncsu.edu.

Abstract

Manipulation of genomic sequences facilitates the identification and characterization of key genetic determinants in the investigation of biological processes. Genome editing via clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated (Cas) constitutes a next-generation method for programmable and high-throughput functional genomics. CRISPR-Cas systems are readily reprogrammed to induce sequence-specific DNA breaks at target loci, resulting in fixed mutations via host-dependent DNA repair mechanisms. Although bacterial genome editing is a relatively unexplored and underrepresented application of CRISPR-Cas systems, recent studies provide valuable insights for the widespread future implementation of this technology. This review summarizes recent progress in bacterial genome editing and identifies fundamental genetic and phenotypic outcomes of CRISPR targeting in bacteria, in the context of tool development, genome homeostasis, and DNA repair.

KEYWORDS:

CRISPR–Cas; Cas9; genome editing

PMID:
25698413
DOI:
10.1016/j.tim.2015.01.008
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center