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Methods Mol Biol. 2015;1275:127-42. doi: 10.1007/978-1-4939-2365-6_9.

MultiPLX: automatic grouping and evaluation of PCR primers.

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Department of Bioinformatics, University of Tartu, Tartu, Estonia.


In this chapter we describe MultiPLX-a tool for automatic grouping of PCR primers for multiplexed PCR. Both generic working principle and step-by-step practical procedures with examples are presented. MultiPLX performs grouping by calculating many important interaction levels between the different primer pairs and then distributes primer pairs to groups so that the strength of unwanted interactions is kept below user-defined compatibility level. In addition it can be used to select optimal primer pairs for multiplexing from list of candidates. MultiPLX can be downloaded from Graphical web-based interface to most functions of MultiPLX is available at

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