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Methods Mol Biol. 2015;1271:189-203. doi: 10.1007/978-1-4939-2330-4_13.

High-resolution atomic force microscopy imaging of rhodopsin in rod outer segment disk membranes.

Author information

1
Institute of Biochemistry and Molecular Medicine, University of Bern, B├╝hlstrasse 28, Bern, CH-3012, Switzerland.

Abstract

Atomic force microscopy (AFM) is a powerful imaging technique that allows recording topographical information of membrane proteins under near-physiological conditions. Remarkable results have been obtained on membrane proteins that were reconstituted into lipid bilayers. High-resolution AFM imaging of native disk membranes from vertebrate rod outer segments has unveiled the higher-order oligomeric state of the G protein-coupled receptor rhodopsin, which is highly expressed in disk membranes. Based on AFM imaging, it has been demonstrated that rhodopsin assembles in rows of dimers and paracrystals and that the rhodopsin dimer is the fundamental building block of higher-order structures.

PMID:
25697525
DOI:
10.1007/978-1-4939-2330-4_13
[Indexed for MEDLINE]

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