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Rev Argent Microbiol. 2015 Jan-Mar;47(1):4-8. doi: 10.1016/j.ram.2014.12.004. Epub 2015 Feb 17.

Development of an enhanced bovine viral diarrhea virus subunit vaccine based on E2 glycoprotein fused to a single chain antibody which targets to antigen-presenting cells.

Author information

1
Instituto de Virología, CICVyA, INTA Castelar, Buenos Aires, Argentina. Electronic address: pecora.andrea@inta.gob.ar.
2
Instituto de Virología, CICVyA, INTA Castelar, Buenos Aires, Argentina.
3
Vetanco S.A., Buenos Aires, Argentina.
4
Algenex SL, Madrid, Spain.

Abstract

Bovine viral diarrhea virus (BVDV) is an important cause of economic losses worldwide. E2 is an immunodominant protein and a promising candidate to develop subunit vaccines. To improve its immunogenicity, a truncated E2 (tE2) was fused to a single chain antibody named APCH, which targets to antigen-presenting cells. APCH-tE2 and tE2 proteins were expressed in the baculovirus system and their immunogenicity was firstly compared in guinea pigs. APCH-tE2 vaccine was the best one to evoke a humoral response, and for this reason, it was selected for a cattle vaccination experiment. All the bovines immunized with 1.5 μg of APCH-tE2 developed high levels of neutralizing antibodies against BVDV up to a year post-immunization, demonstrating its significant potential as a subunit vaccine. This novel vaccine is undergoing scale-up and was transferred to the private sector. Nowadays, it is being evaluated for registration as the first Argentinean subunit vaccine for cattle.

KEYWORDS:

Anticuerpo de cadena simple; Baculovirus; Bovine viral diarrhea virus; E2 glycoprotein; Glucoproteína E2; Single chain antibody; Subunit vaccine; Vacuna a subunidad; Virus de la diarrea viral bovina

PMID:
25697468
DOI:
10.1016/j.ram.2014.12.004
[Indexed for MEDLINE]
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