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Molecules. 2015 Feb 17;20(2):3463-78. doi: 10.3390/molecules20023463.

Nkrp1 family, from lectins to protein interacting molecules.

Author information

1
Institute of Microbiology, v.v.i., Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 414220, Czech Republic. rozbesky@gmail.com.
2
Department of Biochemistry, Faculty of Science, Charles University, Hlavova 8, Prague 212843, Czech Republic. rozbesky@gmail.com.
3
Institute of Microbiology, v.v.i., Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 414220, Czech Republic. ivanova@biomed.cas.cz.
4
Institute of Microbiology, v.v.i., Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 414220, Czech Republic. valeria.grobarova@natur.cuni.cz.
5
Department of Cell Biology, Faculty of Science, Charles University, Viničná 7, Prague 212843, Czech Republic. valeria.grobarova@natur.cuni.cz.
6
Institute of Microbiology, v.v.i., Academy of Sciences of the Czech Republic, Vídeňská 1083, Prague 414220, Czech Republic. pnovak@biomed.cas.cz.
7
Department of Biochemistry, Faculty of Science, Charles University, Hlavova 8, Prague 212843, Czech Republic. pnovak@biomed.cas.cz.

Abstract

The C-type lectin-like receptors include the Nkrp1 protein family that regulates the activity of natural killer (NK) cells. Rat Nkrp1a was reported to bind monosaccharide moieties in a Ca2+-dependent manner in preference order of GalNac > GlcNAc >> Fuc >> Gal > Man. These findings established for rat Nkrp1a have been extrapolated to all additional Nkrp1 receptors and have been supported by numerous studies over the past two decades. However, since 1996 there has been controversy and another article showed lack of interactions with saccharides in 1999. Nevertheless, several high affinity saccharide ligands were synthesized in order to utilize their potential in antitumor therapy. Subsequently, protein ligands were introduced as specific binders for Nkrp1 proteins and three dimensional models of receptor/protein ligand interaction were derived from crystallographic data. Finally, for at least some members of the NK cell C-type lectin-like proteins, the "sweet story" was impaired by two reports in recent years. It has been shown that the rat Nkrp1a and CD69 do not bind saccharide ligands such as GlcNAc, GalNAc, chitotetraose and saccharide derivatives (GlcNAc-PAMAM) do not directly and specifically influence cytotoxic activity of NK cells as it was previously described.

PMID:
25690298
PMCID:
PMC6272133
DOI:
10.3390/molecules20023463
[Indexed for MEDLINE]
Free PMC Article

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