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J Tissue Eng Regen Med. 2017 Apr;11(4):977-988. doi: 10.1002/term.1997. Epub 2015 Feb 17.

Enhanced restoration of in situ-damaged hairs by intradermal transplantation of trichogenous dermal cells.

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PhoenixBio Co. Ltd, Hiroshima, Japan.
Synthetic Biology Research Centre, Department of Hepatology, Graduate School of Medicine, Osaka City University, Japan.


We developed a nude rat model for determining the capacity of trichogenous cells to restore in vivo-damaged hair follicles (HFs). A surgical scalpel was inserted into the rat's dermis to generate the in vivo-damaged pelage HFs, the HFs whose lower parts were lost, but the upper parts containing sebaceous and bulge regions remained intact. Dermal papilla cells (DPCs) and dermal sheath cells (DSCs) from EGFP transgenic rat vibrissae were propagated in culture, and each alone (DPC or DSC) or a mixture (DPC/DSC) was transplanted into the intradermal path made by a scalpel. It was found that the in vivo-damaged HFs had hair self-restoration ability, and the transplanted trichogenic dermal cells prominently enhanced this ability, DPC/DSC transplants being more effective in enhancement than DPC or DSC alone. The restored bulbs contained EGFP-positive cells, shed their original straight shafts, generated new shafts, and further developed into hairs with a sebaceous gland and bulge structures by ~6 weeks post-transplantation. Compared to the preceding animal models, this model is less invasive, requires fewer donor cells and allows repeated operations with higher reproducibility and accuracy. The present study suggests that conditions causing in situ-damaged HFs, such as androgenic alopecia, in which HFs are damaged and miniaturized, can be restored by functional trichogenous dermal cell transplantation therapy.


cell therapy; cell transplantation; dermal papilla cells; dermal sheath cells; epithelial-mesenchymal interaction; hair induction; hair restoration

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