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Biotechnol Bioeng. 2015 Jul;112(7):1488-93. doi: 10.1002/bit.25539. Epub 2015 Apr 8.

Annotation of additional evolutionary conserved microRNAs in CHO cells from updated genomic data.

Author information

1
Department of Biotechnology, BOKU University of Natural Resources and Life Sciences, Vienna, Austria. andreas.diendorfer@boku.ac.at.
2
Department of Biotechnology, BOKU University of Natural Resources and Life Sciences, Vienna, Austria.
3
ACIB GmbH, Austrian Centre of Industrial Biotechnology, Graz, Austria.
4
Biberach University of Applied Sciences, Biberach, Germany.

Abstract

MicroRNAs are small non-coding RNAs that play a critical role in post-transcriptional control of gene expression. Recent publications of genomic sequencing data from the Chinese Hamster (CGR) and Chinese hamster ovary (CHO) cells provide new tools for the discovery of novel miRNAs in this important production system. Version 20 of the miRNA registry miRBase contains 307 mature miRNAs and 200 precursor sequences for CGR/CHO. We searched for evolutionary conserved miRNAs from miRBase v20 in recently published genomic data, derived from Chinese hamster and CHO cells, to further extend the list of known miRNAs. With our approach we could identify several hundred miRNA sequences in the genome. For several of these, the expression in CHO cells could be verified from multiple next-generation sequencing experiments. In addition, several hundred unexpressed miRNAs are awaiting further confirmation by testing for their transcription in different Chinese hamster tissues.

KEYWORDS:

Chinese hamster ovary cell; microRNA; next-generation sequencing

PMID:
25689160
PMCID:
PMC4949662
DOI:
10.1002/bit.25539
[Indexed for MEDLINE]
Free PMC Article

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