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Biopreserv Biobank. 2015 Feb;13(1):56-60. doi: 10.1089/bio.2014.0090.

Comparison of six different pretreatment methods for blood RNA extraction.

Author information

1
1 China National Genebank, BGI-Shenzhen , Shenzhen, China .

Abstract

Human blood specimens serve as important research materials in the field of translational medicine research. The RNA extracted from blood, for example, represents the gene expression profiles of individuals or groups, and can be indicative of the pathological basis for human diseases. Meanwhile, the RNA quality may have severe impacts on the results of RNA studies. RNA is susceptible to many factors, such as the time of sample collection, transportation conditions, protectants, pretreatments, and extraction methods. In this study, six different pretreatment methods are evaluated for their effects on blood RNA extraction including the RNA yields and quality. Results show that most of these methods meet the basic requirements for RNA studies. While considering the simplicity of the procedure, the cost factor, and how to make full use of the samples, the proper method should be employed by researchers who have specific requirements for their research.

PMID:
25686049
DOI:
10.1089/bio.2014.0090
[Indexed for MEDLINE]

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