Format

Send to

Choose Destination
Mol Metab. 2014 Dec 26;4(2):106-17. doi: 10.1016/j.molmet.2014.12.009. eCollection 2015 Feb.

A gut-brain neural circuit controlled by intestinal gluconeogenesis is crucial in metabolic health.

Author information

1
Institut National de la Santé et de la Recherche Médicale, U855, Lyon, F-69008, France ; Université de Lyon, Lyon, F-69008, France ; Université Lyon1, Villeurbanne, F-69622, France.

Abstract

OBJECTIVES:

Certain nutrients positively regulate energy homeostasis via intestinal gluconeogenesis (IGN). The objective of this study was to evaluate the impact of a deficient IGN in glucose control independently of nutritional environment.

METHODS:

We used mice deficient in the intestine glucose-6 phosphatase catalytic unit, the key enzyme of IGN (I-G6pc (-/-) mice). We evaluated a number of parameters involved in energy homeostasis, including insulin sensitivity (hyperinsulinemic euglycaemic clamp), the pancreatic function (insulin secretion in vivo and in isolated islets) and the hypothalamic homeostatic function (leptin sensitivity).

RESULTS:

Intestinal-G6pc (-/-) mice exhibit slight fasting hyperglycaemia and hyperinsulinemia, glucose intolerance, insulin resistance and a deteriorated pancreatic function, despite normal diet with no change in body weight. These defects evoking type 2 diabetes (T2D) derive from the basal activation of the sympathetic nervous system (SNS). They are corrected by treatment with an inhibitor of α-2 adrenergic receptors. Deregulation in a key target of IGN, the homeostatic hypothalamic function (highlighted here through leptin resistance) is a mechanistic link. Hence the leptin resistance and metabolic disorders in I-G6pc (-/-) mice are corrected by rescuing IGN by portal glucose infusion. Finally, I-G6pc (-/-) mice develop the hyperglycaemia characteristic of T2D more rapidly under high fat/high sucrose diet.

CONCLUSIONS:

Intestinal gluconeogenesis is a mandatory function for the healthy neural control of glucose homeostasis.

KEYWORDS:

Autonomous nervous system; Hypothalamus; Insulin secretion; Insulin sensitivity; Intestinal gluconeogenesis; Type 2 diabetes

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center