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Mol Plant. 2015 Jul;8(7):1069-89. doi: 10.1016/j.molp.2015.02.004. Epub 2015 Feb 13.

A Gene Expression Profiling of Early Rice Stamen Development that Reveals Inhibition of Photosynthetic Genes by OsMADS58.

Author information

1
State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China.
2
Center for Quantitative Biology, Peking University, Beijing 100871, China; School of Mathematical Sciences, Peking University, Beijing 100871, China.
3
State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China.
4
State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing 100871, China; Center for Bioinformatics, Peking University, Beijing 100871, China.
5
Center for Quantitative Biology, Peking University, Beijing 100871, China; The National Center of Plant Gene Research, Beijing 100871, China; State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, 624 Jin-Guang Life Science Building, 5 Yiheyuan Road, Beijing 100871, China. Electronic address: shunongb@pku.edu.cn.

Abstract

Stamen is a unique plant organ wherein germ cells or microsporocytes that commit to meiosis are initiated from somatic cells during its early developmental process. While genes determining stamen identity are known according to the ABC model of floral development, little information is available on how these genes affect germ cell initiation. By using the Affymetrix GeneChip Rice Genome Array to assess 51 279 transcripts, we established a dynamic gene expression profile (GEP) of the early developmental process of rice (Oryza sativa) stamen. Systematic analysis of the GEP data revealed novel expression patterns of some developmentally important genes including meiosis-, tapetum-, and phytohormone-related genes. Following the finding that a substantial amount of nuclear genes encoding photosynthetic proteins are expressed at the low levels in early rice stamen, through the ChIP-seq analysis we found that a C-class MADS box protein, OsMADS58, binds many nuclear-encoded genes participated in photosystem and light reactions and the expression levels of most of them are increased when expression of OsMADS58 is downregulated in the osmads58 mutant. Furthermore, more pro-chloroplasts are observed and increased signals of reactive oxygen species are detected in the osmads58 mutant anthers. These findings implicate a novel link between stamen identity determination and hypoxia status establishment.

KEYWORDS:

early stamen development; fate change; gene expression profile; organ identity genes; redox status; rice

PMID:
25684654
DOI:
10.1016/j.molp.2015.02.004
[Indexed for MEDLINE]
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