Format

Send to

Choose Destination
Stem Cell Reports. 2015 Mar 10;4(3):473-88. doi: 10.1016/j.stemcr.2015.01.007. Epub 2015 Feb 13.

Comparative quantification of the surfaceome of human multipotent mesenchymal progenitor cells.

Author information

1
Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, UK.
2
Faculty of Medical and Human Sciences, University of Manchester, Manchester M13 9PT, UK.
3
Faculty of Engineering and Physical Sciences, University of Manchester, Manchester M13 9PT, UK.
4
Faculty of Life Sciences, University of Manchester, Manchester M13 9PT, UK. Electronic address: cay.kielty@manchester.ac.uk.
5
Faculty of Medical and Human Sciences, University of Manchester, Manchester M13 9PT, UK. Electronic address: ann.canfield@manchester.ac.uk.

Abstract

Mesenchymal progenitor cells have great therapeutic potential, yet incomplete characterization of their cell-surface interface limits their clinical exploitation. We have employed subcellular fractionation with quantitative discovery proteomics to define the cell-surface interface proteome of human bone marrow mesenchymal stromal/stem cells (MSCs) and human umbilical cord perivascular cells (HUCPVCs). We compared cell-surface-enriched fractions from MSCs and HUCPVCs (three donors each) with adult mesenchymal fibroblasts using eight-channel isobaric-tagging mass spectrometry, yielding relative quantification on >6,000 proteins with high confidence. This approach identified 186 upregulated mesenchymal progenitor biomarkers. Validation of 10 of these markers, including ROR2, EPHA2, and PLXNA2, confirmed upregulated expression in mesenchymal progenitor populations and distinct roles in progenitor cell proliferation, migration, and differentiation. Our approach has delivered a cell-surface proteome repository that now enables improved selection and characterization of human mesenchymal progenitor populations.

PMID:
25684225
PMCID:
PMC4375938
DOI:
10.1016/j.stemcr.2015.01.007
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center