Format

Send to

Choose Destination
Mol Cell. 2015 Mar 19;57(6):971-983. doi: 10.1016/j.molcel.2015.01.009. Epub 2015 Feb 12.

The methyl-CpG-binding protein MBD7 facilitates active DNA demethylation to limit DNA hyper-methylation and transcriptional gene silencing.

Author information

1
Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China; Department of Horticulture & Landscape Architecture, Purdue University, West Lafayette, IN 47906, USA.
2
Department of Horticulture & Landscape Architecture, Purdue University, West Lafayette, IN 47906, USA.
3
Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
4
Department of Horticulture & Landscape Architecture, Purdue University, West Lafayette, IN 47906, USA; Biotechnology Section, Directorate of Rice Research, Hyderabad 500030, India.
5
Department of Horticulture & Landscape Architecture, Purdue University, West Lafayette, IN 47906, USA; School of Life Sciences, Chongqing University, Chongqing 400044, China.
6
Department of Horticulture & Landscape Architecture, Purdue University, West Lafayette, IN 47906, USA; Department of Horticulture, Zhejiang University, Hangzhou 310058, China.
7
Department of Biochemistry, Purdue University, West Lafayette, IN 47907, USA.
8
State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China.
9
Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China; Department of Horticulture & Landscape Architecture, Purdue University, West Lafayette, IN 47906, USA. Electronic address: jkzhu@purdue.edu.

Abstract

DNA methylation is a conserved epigenetic mark that plays important roles in plant and vertebrate development, genome stability, and gene regulation. Canonical Methyl-CpG-binding domain (MBD) proteins are important interpreters of DNA methylation that recognize methylated CG sites and recruit chromatin remodelers, histone deacetylases, and histone methyltransferases to repress transcription. Here, we show that Arabidopsis MBD7 and Increased DNA Methylation 3 (IDM3) are anti-silencing factors that prevent gene repression and DNA hypermethylation. MBD7 preferentially binds to highly methylated, CG-dense regions and physically associates with other anti-silencing factors, including the histone acetyltransferase IDM1 and the alpha-crystallin domain proteins IDM2 and IDM3. IDM1 and IDM2 were previously shown to facilitate active DNA demethylation by the 5-methylcytosine DNA glycosylase/lyase ROS1. Thus, MBD7 tethers the IDM proteins to methylated DNA, which enables the function of DNA demethylases that in turn limit DNA methylation and prevent transcriptional gene silencing.

PMID:
25684209
PMCID:
PMC4369450
DOI:
10.1016/j.molcel.2015.01.009
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Elsevier Science Icon for PubMed Central
Loading ...
Support Center