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Mech Dev. 2015 May;136:53-63. doi: 10.1016/j.mod.2015.02.002. Epub 2015 Feb 12.

Hematopoietic activity in putative mouse primordial germ cell populations.

Author information

1
Department of Biomedicine and Prevention, Section of Histology and Embryology, University of Rome "Tor Vergata", Rome, Italy.
2
Research Center Ospedale Pediatrico Bambino Gesù, IRCSS, Laboratory of Flow-Cytometry and B Cell Development, Rome, Italy.
3
Department of Biomedicine and Prevention, Section of Histology and Embryology, University of Rome "Tor Vergata", Rome, Italy. Electronic address: defelici@uniroma2.it.

Abstract

In the present paper, starting from the observation of heterogeneous expression of the GOF-18ΔPE-GFP Pou5f1 (Oct3/4) transgene in putative mouse PGC populations settled in the aorta-gonad-mesonephros (AGM) region, we identified various OCT3/4 positive populations showing distinct expression of PGC markers (BLIMP-1, AP, TG-1, STELLA) and co-expressing several proteins (CD-34, CD-41, FLK-1) and genes (Brachyury, Hox-B4, Scl/Tal-1 and Gata-2) of hematopoietic precursors. Moreover, we found that Oct3/4-GFP(weak) CD-34(weak/high) cells possess robust hematopoietic colony forming activity (CFU) in vitro. These data indicate that the cell population usually considered PGCs moving toward the gonadal ridges encompasses a subset of cells co-expressing several germ cell and hematopoietic markers and possessing hematopoietic activity. These results are discussed within of the current model of germline segregation.

KEYWORDS:

Allantois; Germline; Hematopoiesis; Oct3/4; Primordial germ cells

PMID:
25684074
DOI:
10.1016/j.mod.2015.02.002
[Indexed for MEDLINE]
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