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Atherosclerosis. 2015 Apr;239(2):318-21. doi: 10.1016/j.atherosclerosis.2015.02.005. Epub 2015 Feb 7.

Dectin-1 deficiency does not affect atherosclerosis development in mice.

Author information

1
Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands. Electronic address: k.szilagyi@sanquin.nl.
2
Department of Medical Biochemistry, Experimental Vascular Biology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands; Department of Pathology and Department of Molecular Genetics, CARIM, Cardiovascular Research Institute Maastricht, Maastricht University, Maastricht, The Netherlands.
3
Department of Medical Biochemistry, Experimental Vascular Biology, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
4
Tytgat Institute for Liver and Intestinal Research, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.
5
Department of Molecular Cell Biology and Immunology, VU University Medical Center, Amsterdam, The Netherlands.
6
Department of Blood Cell Research, Sanquin Research and Landsteiner Laboratory, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands.

Abstract

OBJECTIVE:

Recent data suggest the involvement of dectin-1 in atherosclerosis through regulation of local reactive oxygen species production. The aim of the current study was to assess the effect of dectin-1 deficiency on atherosclerotic plaque development.

METHODS:

Using immunohistochemistry dectin-1 expression was observed on foamy macrophages in atherosclerotic lesions in mice. Following lethal irradiation LDLR(-/-) mice were reconstituted with bone marrow from either wild type or dectin-1(-/-) mice. After recovery, mice were fed a high fat diet for 9 weeks and atherosclerotic lesions were analyzed.

RESULTS AND CONCLUSION:

Overall, we found no significant differences in plaque size or severity between the groups. Also no differences were observed in granulocyte or macrophage composition of the plaques or in the ability to produce reactive oxygen species by macrophages from both groups. Dectin-1 is dispensable for the development of atherosclerotic lesions in mice.

KEYWORDS:

Atherosclerosis; Dectin-1; Macrophages; Respiratory burst

[Indexed for MEDLINE]
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