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Biochem Biophys Res Commun. 2015 Mar 13;458(3):584-589. doi: 10.1016/j.bbrc.2015.02.009. Epub 2015 Feb 11.

Involvement of the histamine H1 receptor in the regulation of sympathetic nerve activity.

Author information

1
Department of Pharmacology, Hirosaki University, Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, 036-8562, Japan. Electronic address: mmura0123@hotmail.co.jp.
2
Department of Pharmacology, Tohoku University School of Medicine, Seiryo-machi 2-1, Aoba-Ku, Sendai, 980-8575, Japan.
3
Department of Cellphysiology, Akita University School of Medicine, Akita, 010-8543, Japan.
4
Department of Dermatology, Hirosaki University, Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, 036-8562, Japan.
5
Division for Identification and Analysis of Bioactive Peptides, University of Miyazaki, 5200 Kihara, Kiyotake, Miyazaki, 889-1692, Japan.
6
Department of Molecular Pharmacology, Tohoku University School of Medicine, Seiryo-machi 2-1, Aoba-Ku, Sendai, 980-8575, Japan.
7
Social Medicine, Hirosaki University, Graduate School of Medicine, 5 Zaifu-cho, Hirosaki, 036-8562, Japan.

Abstract

The histamine system is involved in the regulation of the autonomic nervous system. We used gene-targeted mice to investigate the role of histamine receptors in the regulation of the sympathetic nervous system. Reverse transcription-polymerase chain reaction (RT-PCR) analysis revealed histamine H1, H2, and H3 receptor expression in the superior cervical ganglion, which contains sympathetic nerve cell bodies. We measured the heart rate variability (HRV), the changes in the beat-to-beat heart rate, which is widely used to assess autonomic activity in the heart. H1 blockade attenuated the baroreflex-mediated changes in heart rate in wild-type (WT) mice, whereas the heart rate response to H2- and H3-specific blockers was unaffected. l-Histidine decarboxylase (HDC) expression in the superior cervical ganglion of H1R-null mice was higher than that in WT controls, whereas the enzyme levels in H2R- and H3R-null mice were not significantly different from those in the WT. All mutant mice (H1R-, H2R-, and H3R-null mice) showed normal electrocardiogram (ECG) patterns with little modification in ECG parameters and the expected response to the β-adrenergic blocker propranolol. Similar to our findings in WT mice, H1 blockade attenuated the baroreflex-mediated heart rate change in H1R-null mice, whereas the heart rate response was unaffected in H2R- and H3R-null mice. The HRV analysis revealed relatively unstable RR intervals, an increased standard deviation of the interbeat interval (SDNN), and low-frequency (LF) component in H1R-null mice compared with the other groups, suggesting that sympathetic nerve activity was altered in H1R-null mice. Taken together, our findings indicate that H1 receptors play a major role in the regulation of sympathetic nerve activity.

KEYWORDS:

Autonomic nerves; ECG; Histamine; Mouse; Sympathetic nerves

PMID:
25680462
DOI:
10.1016/j.bbrc.2015.02.009
[Indexed for MEDLINE]

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