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Cell. 2015 Feb 12;160(4):619-630. doi: 10.1016/j.cell.2015.01.032.

Phosphatidylserine vesicles enable efficient en bloc transmission of enteroviruses.

Author information

1
Laboratory of Host-Pathogen Dynamics, National Heart Lung and Blood Institute, NIH, Bethesda, MD 20892, USA; Federated Department of Biological Sciences, Rutgers University, Newark, NJ 07102, USA.
2
Laboratory of Host-Pathogen Dynamics, National Heart Lung and Blood Institute, NIH, Bethesda, MD 20892, USA.
3
Federated Department of Biological Sciences, Rutgers University, Newark, NJ 07102, USA.
4
Electron Microscopy Core Facility, National Heart Lung and Blood Institute, NIH, Bethesda, MD 20892, USA.
5
Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, NY 11794, USA.
6
Program in Computational Biology and Immunology, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
7
Laboratory of Host-Pathogen Dynamics, National Heart Lung and Blood Institute, NIH, Bethesda, MD 20892, USA. Electronic address: nihal.altan-bonnet@nih.gov.

Abstract

A central paradigm within virology is that each viral particle largely behaves as an independent infectious unit. Here, we demonstrate that clusters of enteroviral particles are packaged within phosphatidylserine (PS) lipid-enriched vesicles that are non-lytically released from cells and provide greater infection efficiency than free single viral particles. We show that vesicular PS lipids are co-factors to the relevant enterovirus receptors in mediating subsequent infectivity and transmission, in particular to primary human macrophages. We demonstrate that clustered packaging of viral particles within vesicles enables multiple viral RNA genomes to be collectively transferred into single cells. This study reveals a novel mode of viral transmission, where enteroviral genomes are transmitted from cell-to-cell en bloc in membrane-bound PS vesicles instead of as single independent genomes. This has implications for facilitating genetic cooperativity among viral quasispecies as well as enhancing viral replication.

PMID:
25679758
PMCID:
PMC6704014
DOI:
10.1016/j.cell.2015.01.032
[Indexed for MEDLINE]
Free PMC Article

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