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Sci Rep. 2015 Feb 13;5:8461. doi: 10.1038/srep08461.

An ESCRT module is required for neuron pruning.

Author information

1
MRC Centre for Developmental Neurobiology, King's College London, London, SE1 1UL.
2
Department of Infectious Diseases, Second Floor Borough Wing, Guy's Hospital, London, SE1 9RT.

Abstract

Neural circuits are refined by both functional and structural changes. Structural remodeling by large-scale pruning occurs where relatively long neuronal branches are cut away from their parent neuron and removed by local degeneration. Until now, the molecular mechanisms executing such branch severing events have remained poorly understood. Here, we reveal a role for the Endosomal Sorting Complex Required for Transport (ESCRT) machinery during neuronal remodeling. Our data show that a specific ESCRT pruning module, including members of the ESCRT-I and ESCRT-III complexes, but not ESCRT-0 or ESCRT-II, are required for the neurite scission event during pruning. Furthermore we show that this ESCRT module requires a direct, in vivo, interaction between Shrub/CHMP4B and the accessory protein Myopic/HD-PTP.

PMID:
25676218
PMCID:
PMC4327575
DOI:
10.1038/srep08461
[Indexed for MEDLINE]
Free PMC Article

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