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PLoS One. 2015 Feb 11;10(2):e0117682. doi: 10.1371/journal.pone.0117682. eCollection 2015.

Generation of a felinized swine endothelial cell line by expression of feline decay-accelerating factor.

Author information

1
Division of Regenerative Medicine, The Jikei University School of Medicine, Tokyo, Japan.
2
Department of Anatomy, The Jikei University School of Medicine, Tokyo, Japan.
3
Division of Organ Transplantation, Department of Regenerative Medicine, Osaka University Graduate School of Medicine, Osaka, Japan.
4
Laboratory of Small Animal Surgery I, School of Veterinary Medicine, Kitasato University, Aomori, Japan.
5
Meiji University International Institute for Bio-Resource Research, Kawasaki, Japan.
6
Division of Regenerative Medicine, The Jikei University School of Medicine, Tokyo, Japan; Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan.
7
Division of Nephrology and Hypertension, Department of Internal Medicine, The Jikei University School of Medicine, Tokyo, Japan.

Abstract

Embryonic stem cell research has facilitated the generation of many cell types for the production of tissues and organs for both humans and companion animals. Because ≥30% of pet cats suffer from chronic kidney disease (CKD), xenotransplantation between pigs and cats has been studied. For a successful pig to cat xenotransplant, the immune reaction must be overcome, especially hyperacute rejection. In this study, we isolated the gene for feline decay-accelerating factor (fDAF), an inhibitor of complement proteins, and transfected a swine endothelial cell line with fDAF to "felinize" the pig cells. These fDAF-expressing cells were resistant to feline serum containing anti-pig antibodies, suggesting that felinized pig cells were resistant to hyperacute rejection. Our results suggest that a "felinized" pig kidney can be generated for the treatment of CKD in cats in the future.

PMID:
25671605
PMCID:
PMC4324824
DOI:
10.1371/journal.pone.0117682
[Indexed for MEDLINE]
Free PMC Article

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